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Dommers S, Heinlein UA. Recombinant polypeptides transplanted from pUMA vector-derived cRNAs are translocated by microsomal membranes and exported out of frog oocytes.
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Vector made for in vitro transcription of cloned inserts encoding open up reading frames. Transcribed RNA encodes the murine NCAM signal sequence for protein export and can comprise the authentic 5'-UTR of X. laevis beta globin over the 5' stop.